【公開日：2025.06.10】【最終更新日：2025.05.13】
課題データ / Project Data
課題番号 / Project Issue Number
24UT0104
利用課題名 / Title
Analysis of microbial cells
利用した実施機関 / Support Institute
東京大学 / Tokyo Univ.
機関外・機関内の利用 / External or Internal Use
外部利用/External Use
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）計測・分析/Advanced Characterization（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）革新的なエネルギー変換を可能とするマテリアル/Materials enabling innovative energy conversion（副 / Sub）次世代バイオマテリアル/Next-generation biomaterials
キーワード / Keywords
質量分析/ Mass spectrometry,環境発電/ Energy Harvesting,生体イメージング/ In vivo imaging,バイオアダプティブ材料/ Bioadaptive materials,細胞培養デバイス/ Cell Culture Device
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
McGlynn Shawn
所属名 / Affiliation
東京科学大学地球生命研究所
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
Andrew Utada
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）技術補助/Technical Assistance（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
Quantitative single-cell resolved microscopy is being utilized to understand growth:energy relationships at a level hitherto un-achieved. We have resolved cell division events and the material contributions to these by quantitatively tracking material accumulation. These results are integrated into metabolic models to determine growth yields, maintenance, etc.
実験 / Experimental
Cells are grown on pads with time lapse resolution, then prepared for NanoSIMS 50L analysis. NanoSIMS is performed as described in McGlynn et al. 2015 Nature.
結果と考察 / Results and Discussion
We have analyzed hundreds of cells and demonstrated the viability of our technique. Specifically, cells can be monitored using light microscopy, then prepared in a way that nanoSIMS analysis is possible. A key deficiency in the current preparation protocol is the loss of cells between stages of preparation, which will be overcome by modifying the current procedure.
図・表・数式 / Figures, Tables and Equations
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
Single cell activity reveals direct electron transfer in methanotrophic consortia. SE McGlynn, GL Chadwick, CP Kempes, and VJ Orphan. Nature, 2015 526, 531–535.
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件