【公開日：2025.06.10】【最終更新日：2025.04.18】
課題データ / Project Data
課題番号 / Project Issue Number
24NU0425
利用課題名 / Title
Application photosynthesis for post sewage treatment of microbial fuel cell
利用した実施機関 / Support Institute
名古屋大学 / Nagoya Univ.
機関外・機関内の利用 / External or Internal Use
外部利用/External Use
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）物質・材料合成プロセス/Molecule & Material Synthesis（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）その他/Others（副 / Sub）-
キーワード / Keywords
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
吉田 奈央子
所属名 / Affiliation
名古屋工業大学社会工学科
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
Que Nguyen Ho
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
林 育生
利用形態 / Support Type
（主 / Main）技術代行/Technology Substitution（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
In this study, the system was implemented in wastewater treatment plants and consisted of two 50-L photosynthesis reactors, labeled F1 and F2, operated under identical conditions. The photosynthesis process was utilized to remove nitrogen and organic matter from sewage. As a result, photosynthetic microorganisms played a crucial role in eliminating these contaminants. The removal process occurred through assimilation. Therefore, to examine the assimilation of carbon (C), hydrogen (H), and nitrogen (N) by photosynthetic microorganisms, CHN analysis was conducted. Biomass samples were collected from the fiber every two weeks.
実験 / Experimental
The biomass was collected from photosynthesis reactors. After collection, the samples were centrifuged and stored at –20°C until analysis. Prior to analysis, the samples were frozen in liquid nitrogen and then milled into a fine powder at Nagoya Institute Technology University. After that, the powdered samples were sent to the ARIM at Nagoya University, Japan, for CHN analysis by CHNS/O Elemental Analyzer.
結果と考察 / Results and Discussion
The ultimate analysis was conducted to determine the carbon (C), hydrogen (H), and nitrogen (N) contents in algal biomass, as shown in Table 1. This study primarily focused on N to calculate its recovery rate through concentrated biomass during the photosynthesis process. The N content in the biomass was determined using the molecular equation of the biomass, and the recovery rate was then calculated. To construct the molecular equation, the contents of C, N, and H were derived from measured data. Additional elements, including oxygen (O), phosphorus (P), and sulfur (S), were analyzed using literature data (Picardo, de Medeiros et al. 2013). Notably, the C:H:N ratio showed no significant difference between F1 and F2, leading to the selection of F1 as the representative case for assessing the primary elements stored in the biomass during Phase I and Phase II.The dominant microalgae belonged to the genus Chlamydomonas, with C:H:N ratios of 1:1.79:0.14 and 1:1.76:0.16 in Phase I and Phase II, respectively. These ratios are similar to those reported in a previous study, which determines the molecular formula for the same genus as C:H:N=1:1.91:0.16 (Picardo, de Medeiros et al. 2013). Therefore, based on the measured data and literature, the overall molecular formulas of typical biomass components were determined to be C1H1.79N0.14O0.36P0.011S0.003 in Phase I and C1H1.76N0.16O0.36P0.011S0.003 in Phase II.
図・表・数式 / Figures, Tables and Equations
Table 1. Contents of C, H, and N were detected in both reactors in Phase I and Phase II.
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
Picardo, M. C., et al. (2013). "A methodology for screening of microalgae as a decision making tool for energy and green chemical process applications." Clean Technologies and Environmental Policy 15: 275-291.
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件